Mouse Arfrp1 activation kit by CRISPRa

CAT#: GA210582

Arfrp1 CRISPRa kit - CRISPR gene activation of mouse ADP-ribosylation factor related protein 1

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: Arfrp1
• Locus ID: 76688
• Kit Components:

  GA210582G1, Arfrp1 gRNA vector 1 in pCas-Guide-CRISPRa

  GA210582G2, Arfrp1 gRNA vector 2 in pCas-Guide-CRISPRa

  GA210582G3, Arfrp1 gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001165991, NM_001165992, NM_001165995, NM_029702, NR_028585
• Synonyms: 1500006I01Rik; AI480700
• Summary: The gene encodes a membrane-associated GTPase that is related to the ADP-ribosylation factor (ARF) and ARF-like (ARL) genes. It plays an essential role in Golgi function controlling recruitment of GRIP domain proteins and ARL1 to the trans-Golgi and trans-Golgi to plasma membrane trafficking of cell surface proteins such as E-cadherin. Deletion of this gene in mice leads to embryonic lethality during early gastrulation, which is at least partly caused by the disruption of E-cadherin trafficking to the cell surface and therefore lack of sufficient cell-cell adhesion in the embryo. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]