Mouse Sacm1l activation kit by CRISPRa

CAT#: GA211232

Sacm1l CRISPRa kit - CRISPR gene activation of mouse SAC1 suppressor of actin mutations 1-like (yeast)

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: Sacm1l
• Locus ID: 83493
• Kit Components:

  GA211232G1, Sacm1l gRNA vector 1 in pCas-Guide-CRISPRa

  GA211232G2, Sacm1l gRNA vector 2 in pCas-Guide-CRISPRa

  GA211232G3, Sacm1l gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_030692, NM_001357485
• Synonyms: mKIAA0851; SAC1; Sac1p
• Summary: This gene encodes an integral membrane protein, which is localized to the endoplasmic reticulum, and functions as a phosphoinositide phosphatase that hydrolyzes phosphatidylinositol 3-phosphate, phosphatidylinositol 4-phosphate, and phosphatidylinositol 3,5-bisphosphate. Deletion of this gene in mouse results in preimplantation lethality. Other studies suggest that this gene is also involved in the organization of golgi membranes and mitotic spindles. Two isoforms are predicted to be produced from the same mRNA by the use of alternative in-frame translation termination codons via a stop codon readthrough mechanism. [provided by RefSeq, Dec 2017]