Mouse Ptges2 activation kit by CRISPRa

CAT#: GA211461

Ptges2 CRISPRa kit - CRISPR gene activation of mouse prostaglandin E synthase 2

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: Ptges2
• Locus ID: 96979
• Kit Components:

  GA211461G1, Ptges2 gRNA vector 1 in pCas-Guide-CRISPRa

  GA211461G2, Ptges2 gRNA vector 2 in pCas-Guide-CRISPRa

  GA211461G3, Ptges2 gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_133783
• Synonyms: 0610038H10Rik; C79137; Gbf1; Mpges2; Pges2
• Summary: The protein encoded by this gene is a Golgi membrane-associated prostaglandin E synthase candidate, which is capable of catalyzing the conversion of prostaglandin H2 to prostaglandin E2 in vitro. However, a study using mice deficient of this gene suggests that this enzyme does not contribute to prostaglandin E2 biosynthesis in vivo. This protein is synthesized as a Golgi membrane-bound protein, but its N-terminal hydrophobic region is cleaved off during protein maturation to produce the predominant soluble truncated form that still retains the enzyme activity. This soluble protein also has been shown to activate the transcription regulated by a gamma-interferon-activated transcription element (GATE), possibly via an interaction with CAAAT/enhancer-binding protein-beta. [provided by RefSeq, Oct 2009]