Mouse Cse1l activation kit by CRISPRa

CAT#: GA212337

Cse1l CRISPRa kit - CRISPR gene activation of mouse chromosome segregation 1-like (S. cerevisiae)

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: Cse1l
• Locus ID: 110750
• Kit Components:

  GA212337G1, Cse1l gRNA vector 1 in pCas-Guide-CRISPRa

  GA212337G2, Cse1l gRNA vector 2 in pCas-Guide-CRISPRa

  GA212337G3, Cse1l gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_023565
• Synonyms: 2610100P18Rik; AA407533; Capts; Cas; Xpo2
• Summary: Export receptor for importin-alpha. Mediates importin-alpha re-export from the nucleus to the cytoplasm after import substrates (cargos) have been released into the nucleoplasm. In the nucleus binds cooperatively to importin-alpha and to the GTPase Ran in its active GTP-bound form. Docking of this trimeric complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the importin-alpha from the export receptor. CSE1L/XPO2 then return to the nuclear compartment and mediate another round of transport. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. [UniProtKB/Swiss-Prot Function]