SMURF 2 (SMURF2) Human Gene Knockout Kit (CRISPR)
CAT#: KN210866RB
SMURF2 - human gene knockout kit via CRISPR, HDR mediated
HDR-mediated knockout kit validation
USD 1,290.00
2 Weeks*
Specifications
Product Data | |
Format | 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control |
Donor DNA | RFP-BSD |
Symbol | SMURF2 |
Locus ID | 64750 |
Disclaimer | The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process. |
Reference Data | |
RefSeq | NM_022739 |
Synonyms | DKFZp686F0270; MGC138150 |
Summary | E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level. [UniProtKB/Swiss-Prot Function] |
Documents
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
KN210866 | SMURF2 - human gene knockout kit via CRISPR, HDR mediated |
USD 1,290.00 |
|
KN210866BN | SMURF2 - human gene knockout kit via CRISPR, HDR mediated |
USD 1,290.00 |
|
KN210866LP | SMURF2 - human gene knockout kit via CRISPR, HDR mediated |
USD 1,290.00 |
|
KN410866 | SMURF2 - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated. |
USD 1,290.00 |
|
GA112118 | SMURF2 CRISPRa kit - CRISPR gene activation of human SMAD specific E3 ubiquitin protein ligase 2 |
USD 1,290.00 |
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