AAV Serotyping Kit II

CAT#: CV500002

This kit contains pre-made AAV-1, AAV-3, AAV-7, AAV-rh.10, AAV-DJ, and AAV-PHP.eB control particles for testing each serotypes infection efficiency in your desired cell type.

Specifications

Product Data

• Promoter: CMV
• Validation: To validate the quality of our AAV control particles, we have developed the following procedure:
  AAV genome titer is determined by qPCR.
  AAV purity is determined by Silver staining.
  AAV transduction efficiency is analyzed by in vitro transduction in HEK293T.
• Vector: pAAV-AC-GFP
• Components: AAV-1 | >10^13 GC/mL | 25uL
  AAV-3 | >10^13 GC/mL | 25uL
  AAV-7 | >10^13 GC/mL | 25uL
  AAV-rh.10 | >10^13 GC/mL | 25uL
  AAV-DJ | >10^13 GC/mL | 25uL
  AAV-PHP.eB | >10^13 GC/mL | 25uL
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, vectors should be aliquoted into single use portions to avoid repeated freeze/thaw cycles.