Renilla luciferase assay kit, 150 assays

CAT#: PR300002

Renilla luciferase assay kit, 150 assays

Specifications

Product Data

• Components: • 5X Passive Lysis Buffer (10 mL)
  • Renilla Luciferase Assay Buffer 2.0 (15 mL)
  • Aquaphile Coelenterazine (3 X 200ug)
• Features: • Highly sensitive & linear flash-type RLuc assay
  • Easy-to-handle water-soluble substrates
  • Excellent performance & great value compared to other suppliers
• Assay Type: Luminescence (flash-type), Endpoint assay
• Detection Method: Luminometer (single-tube or microplate reader with reagent injectors)
• Summary: This Renilla luciferase assay kit is designed to measure Renilla luciferase (RLuc) activity in transfected cell reporter gene assays. This kit utilizes a special buffer formulation designed to yield reliable, linear measurements of Renilla luciferase activity with minimal autoluminescence background and superior sensitivity.The substrate included in the kit is a water soluble substrate, aquaphile Coelenterazine, which can be stored at -20°C with minimal evaporation, unlike methanol solutions of coelenterazine. The stated number of assays is based on 96-well assay format. The kits include enough Passive Lysis Buffer to perform the stated number of assays in plate sizes ranging from 96-well to 24-well. If needed, additional 5X Passive Lysis Buffer (Cat# PR300004) can be purchased separately.
• Note: This kit is a flash-type luminescence assay, which requires luminescence to be measured immediately after adding reagents to your sample.
• Background: Renilla luciferase has been used as a reporter gene for studying gene regulation and function in vitro and in vivo. It commonly is used in multiplex transcriptional reporter assays or as a normalizing transfection control for firefly luciferase assays. Renilla luciferase, a monomeric 36,000 Dalton protein, catalyzes coelenterazine oxidation by oxygen to produce light. The enzyme does not require post-translational modification for its activity and may function as a genetic reporter immediately following translation. Coelenterazine also emits light from enzyme-independent oxidation, a process known as autoluminescence. The autoluminescence is enhanced by superoxide anion and peroxynitrite in cells and tissues. This assay kit utilizes a special buffer formulation designed to yield reliable, linear measurements of Renilla luciferase activity with minimal autoluminescence background and superior sensitivity.
• Storage: Store at -80°C.
• Stability: Renilla Luciferase Assay Buffer 2.0 is stable at -80°C for at least six months from date of receipt. Other kit components are stable at -20°C for at least six months from date of receipt. Kit components and stock solutions of Aquaphile coelenterazine in water are stable to at least 5 freeze/thaw cycles.