Turbo-mRNA Transfectin kit

CAT#: TT100314

Turbo-mRNA Transfection kit (1 vial of 1 mL Turbo-mRNA reagen and 1 vial of 1 mL mRNA Boost reagent)

Product Images

Efficient Genome Editing with Cas9 mRNA and verified gRNA. HEK293T/17, U2OS and NHDF cells were co-transfected with 0.5 µg of Cas9 encoding mRNA, and 25nM verified gRNA using Turbo-mRNA Transfection Kit (0.5 µl/well of 24-well plate of both mRNA Reagent and Boost, OriGene Catalog TT100314). A T7E1 mismatch detection assay was used to measure cleavage efficiency at 48 hours post-transfection.

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  Efficient Genome Editing with Cas9 mRNA and verified gRNA. A defective RFP gene (with deletions) was inserted in the genome of HEK293T cells using CRISPR knockin technology. The RFP negative parent cells were co-transfected with Cas9 encoding mRNA, RFP repairing oligos, and different gRNAs using Turbo-mRNA Transfection Kit (OriGene Catalog TT100314). RFP gene can be repaired by the Cas9 mRNA, verified RFP gRNA and wild type RFP oligos but not scrambled control. RFP positive cells can be detected at 3 days post-transfection. Left: Verified RFP gRNA ; Right: Scrambled gRNA.

Specifications

Product Data

• Components: One tube (1 mL) Turbo-mRNA Transfection Reagent and one tube (1 mL) mRNA Boost reagent
• Quality Control: The Turbo-mRNA Transfection Kit can be used to deliver a variety of RNA molecules including mRNAs and viral RNAs (2–10 kb). It can be used for multiple applications such as short-term protein expression, viral production and replication studies. This kit is compatible with serum; transfection efficiency is optimal when transfections are performed in the presence of serum, with no medium change required.
• Storage: Store both Turbo-mRNA Reagent and mRNA Boost Reagent at 4°C. Before each use, warm to room temperature and vortex gently.
• Stability: The transfection reagent is stable for one (1) year from date of shipping when stored at +4°C.