GFAP CytoSection

CAT#: TS404548P5

Transient overexpression of GFAP (NM_002055) in HEK293T cells paraffin embedded 4 um sections, controls for ICC/IHC staining, 25 slides per pack

Product Images

H&E staining of GFAP overexpressed cytosection (TS404548), HEK293T cells were transfected with cDNA clone RC204548, 4um sections, 10x magnification

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  H&E staining of GFAP overexpressed cytosection (TS404548), HEK293T cells were transfected with cDNA clone RC204548, 4um in thickness, 40x magnification

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  Immunohistology staining on GFAP overexpressed cytosection (TS404548) with mouse anti GFAP antibody (UM500094) at 1:200 dilution, 1hr room temperature. The staining was achieved with HIER Tris/EDTA buffer, pH9.0, 100oC for 20 min, Polink2 detection system (D22-18) with DAB chromogen. HEK293T cells were transfected with cDNA clone RC204548, 4um sections, 40x magnification

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  Immunohistology staining of untransfected HEK293T with mouse anti GFAP antibody (UM500094) at 1:200 dilution, 1 hour at room temperature. The staining was conducted with HIER Tris/EDTA buffer, pH9.0, 100oC for 20 min, Polink2 detection system with DAB chromogen (D22-18). HEK293T cells were 4um sections, 40x magnification.

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  Immunohistology staining on GFAP overexpressed cytosection (TS404548) with mouse anti DDK clone 11C3 C/N TA180144 at 1:1000 dilution 4C O/N. Antibody staining was achieved with HIER Tris/EDTA pH9 for 20minutes, Polink2 detection system with DAB chromogen C/N D22-18. Positive stain shown with the brown chromogen present. HEK293T cells were transfected with cDNA clone RC204548, 4um sections, 40x magnification

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  Immunohistology staining on HEK293T untransfected control section screened with mouse anti-DDK clone 11C3 C/N TA180144 at 1:1000 dilution 4C O/N. Antibody staining was achieved with HIER Tris/EDTA pH9 for 20minutes, Polink2 detection system with DAB chromogen C/N D22-18. No brown chromogen showing negative stain.

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Specifications

Product Data

• note: This product is for research use only and is not approved for use in humans or in clinical diagnosis.
• symbol: GFAP
• families_filter: ES Cell Differentiation/IPS
• tags_filter: C-MYC/DDK
• sub_category: Cytosections
• description: Transient overexpression of GFAP (NM_002055) in HEK293T cells paraffin embedded 4 um sections, controls for ICC/IHC staining, 25 slides per pack
• pdf_url: /datasheet/ts404548p5.pdf
• detection_antibodies: Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
• storage: Room Temperature
• symbol_filter: GFAP
• cytogenetics: 17q21.31
• preparation: HEK293T cells were transiently transfected with TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs. After harvesting, the cultured cells were fixed in formalin & dehydrated before embedding in paraffin. 4 um sections of the FFPE cell pellet blocks are cut and mounted on positively charged SuperFrost slides.
• expression_hosts_filter: HEK293T
• stability: Slides are guaranteed for a year from the date of receipt if proper storage instructions were followed.
• accn: NM_002055, NP_002046
• sds_url: /sds/ts404548p5.pdf
• species_filter: Human
• _pt: 1
• expression_cdna_clone_or_aa_sequence: TrueORF Clone RC204548
• _c: over-expression-cell-pellet
• availabilities_filter: In Stock
• synonyms: ALXDRD
• format: 4 um sections on SuperFrost Slides
• refseq: NP_002046
• families: ES Cell Differentiation/IPS
• product_name: GFAP CytoSection
• accession_number: NM_002055, NP_002046
• target_detection_antibodies: GFAP Mouse Monoclonal Antibody [Clone ID: OTI4G8] (TA500337)
• locus_id: 2670
• category: Proteins
• sizes_filter: 25 Slides