PCMT1 (NM_005389) Human Mass Spec Standard

CAT#: PH300327

PCMT1 MS Standard C13 and N15-labeled recombinant protein (NP_005380)

Product Images

Coomassie blue staining of purified PCMT1 protein (Cat# TP300327). The protein was produced from HEK293T cells transfected with PCMT1 cDNA clone (Cat# RC200327) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC200327
• Predicted MW: 24.7 kDa
• Protein Sequence:
  Sequence Link

  >RC200327 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAWKSGGASHSELIHNLRKNGIIKTDKVFEVMLATDRSHYAKCNPYMDSPQSIGFQATISAPHMHAYALE
  LLFDQLHEGAKALDVGSGSGILTACFARMVGCTGKVIGIDHIKELVDDSINNVRKDDPTLLSSGRVQLVV
  GDGRMGYAEEAPYDAIHVGAAAPVVPQALIDQLKPGGRLILPVGPAGGNQMLEQYDKLQDGSIKMKPLMG
  VIYVPLTDKEKQWSRWK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_005380
• RefSeq Size: 1751
• RefSeq ORF: 681
• Synonyms: PIMT
• Locus ID: 5110
• UniProt ID: P22061, A0A0A0MRJ6
• Cytogenetics: 6q25.1
• Summary: 'This gene encodes a member of the type II class of protein carboxyl methyltransferase enzymes. The encoded enzyme plays a role in protein repair by recognizing and converting D-aspartyl and L-isoaspartyl residues resulting from spontaneous deamidation back to the normal L-aspartyl form. The encoded protein may play a protective role in the pathogenesis of Alzheimer's disease, and single nucleotide polymorphisms in this gene have been associated with spina bifida and premature ovarian failure. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Oct 2011]'
• Protein Families: Druggable Genome