glutathione S transferase Omega 1 (GSTO1) (NM_004832) Human Mass Spec Standard

CAT#: PH300362

GSTO1 MS Standard C13 and N15-labeled recombinant protein (NP_004823)

Product Images

Coomassie blue staining of purified GSTO1 protein (Cat# TP300362). The protein was produced from HEK293T cells transfected with GSTO1 cDNA clone (Cat# RC200362) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC200362
• Predicted MW: 27.6 kDa
• Protein Sequence:
  Sequence Link

  >RC200362 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSGESARSLGKGSAPPGPVPEGSIRIYSMRFCPFAERTRLVLKAKGIRHEVININLKNKPEWFFKKNPFG
  LVPVLENSQGQLIYESAITCEYLDEAYPGKKLLPDDPYEKACQKMILELFSKVPSLVGSFIRSQNKEDYA
  GLKEEFRKEFTKLEEVLTNKKTTFFGGNSISMIDYLIWPWFERLEAMKLNECVDHTPKLKLWMAAMKEDP
  TVSALLTSEKDWQGFLELYLQNSPEACDYGL
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_004823
• RefSeq Size: 1017
• RefSeq ORF: 723
• Synonyms: GSTO 1-1; GSTTLp28; HEL-S-21; P28; SPG-R
• Locus ID: 9446
• UniProt ID: P78417, V9HWG9
• Cytogenetics: 10q25.1
• Summary: The protein encoded by this gene is an omega class glutathione S-transferase (GST) with glutathione-dependent thiol transferase and dehydroascorbate reductase activities. GSTs are involved in the metabolism of xenobiotics and carcinogens. The encoded protein acts as a homodimer and is found in the cytoplasm. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]
• Protein Families: Druggable Genome
• Protein Pathways: Drug metabolism - cytochrome P450, Glutathione metabolism, Metabolism of xenobiotics by cytochrome P450