Cathepsin H (CTSH) (NM_004390) Human Mass Spec Standard

CAT#: PH300373

CTSH MS Standard C13 and N15-labeled recombinant protein (NP_004381)

Product Images

Coomassie blue staining of purified CTSH protein (Cat# TP300373). The protein was produced from HEK293T cells transfected with CTSH cDNA clone (Cat# RC200373) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC200373
• Predicted MW: 37.4 kDa
• Protein Sequence:
  Sequence Link

  >RC200373 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MWATLPLLCAGAWLLGVPVCGAAELSVNSLEKFHFKSWMSKHRKTYSTEEYHHRLQTFASNWRKINAHNN
  GNHTFKMALNQFSDMSFAEIKHKYLWSEPQNCSATKSNYLRGTGPYPPSVDWRKKGNFVSPVKNQGACGS
  CWTFSTTGALESAIAIATGKMLSLAEQQLVDCAQDFNNHGCQGGLPSQAFEYILYNKGIMGEDTYPYQGK
  DGYCKFQPGKAIGFVKDVANITIYDEEAMVEAVALYNPVSFAFEVTQDFMMYRTGIYSSTSCHKTPDKVN
  HAVLAVGYGEKNGIPYWIVKNSWGPQWGMNGYFLIERGKNMCGLAACASYPIPLV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_004381
• RefSeq Size: 1494
• RefSeq ORF: 1005
• Synonyms: ACC-4; ACC-5; ACC4; ACC5; CPSB
• Locus ID: 1512
• UniProt ID: P09668
• Cytogenetics: 15q25.1
• Summary: 'The protein encoded by this gene is a lysosomal cysteine proteinase important in the overall degradation of lysosomal proteins. It is composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. The encoded protein, which belongs to the peptidase C1 protein family, can act both as an aminopeptidase and as an endopeptidase. Increased expression of this gene has been correlated with malignant progression of prostate tumors. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016]'
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Lysosome