POLR2H (NM_006232) Human Mass Spec Standard

CAT#: PH300650

POLR2H MS Standard C13 and N15-labeled recombinant protein (NP_006223)

Product Images

Coomassie blue staining of purified POLR2H protein (Cat# TP300650). The protein was produced from HEK293T cells transfected with POLR2H cDNA clone (Cat# RC200650) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC200650
• Predicted MW: 17.1 kDa
• Protein Sequence:
  Sequence Link

  >RC200650 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAGILFEDIFDVKDIDPEGKKFDRVSRLHCESESFKMDLILDVNIQIYPVDLGDKFRLVIASTLYEDGTL
  DDGEYNPTDDRPSRADQFEYVMYGKVYRIEGDETSTEAATRLSAYVSYGGLLMRLQGDANNLHGFEVDSR
  VYLLMKKLAF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_006223
• RefSeq Size: 1264
• RefSeq ORF: 450
• Synonyms: RPABC3; RPB8; RPB17
• Locus ID: 5437
• UniProt ID: P52434
• Cytogenetics: 3q27.1
• Summary: 'The three eukaryotic RNA polymerases are complex multisubunit enzymes that play a central role in the transcription of nuclear genes. This gene encodes an essential and highly conserved subunit of RNA polymerase II that is shared by the other two eukaryotic DNA-directed RNA polymerases, I and III. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]'
• Protein Families: Transcription Factors
• Protein Pathways: Huntington's disease, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase