Fibrillarin (FBL) (NM_001436) Human Mass Spec Standard

CAT#: PH300732

FBL MS Standard C13 and N15-labeled recombinant protein (NP_001427)

Product Images

Coomassie blue staining of purified FBL protein (Cat# TP300732). The protein was produced from HEK293T cells transfected with FBL cDNA clone (Cat# RC200732) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC200732
• Predicted MW: 33.6 kDa
• Protein Sequence:
  Sequence Link

  >RC200732 representing NM_001436
  Red=Cloning site Green=Tags(s)
  
  MKPGFSPRGGGFGGRGGFGDRGGRGGRGGFGGGRGRGGGFRGRGRGGGGGGGGGGGGGRGGGGFHSGGNR
  GRGRGGKRGNQSGKNVMVEPHRHEGVFICRGKEDALVTKNLVPGESVYGEKRVSISEGDDKIEYRAWNPF
  RSKLAAAILGGVDQIHIKPGAKVLYLGAASGTTVSHVSDIVGPDGLVYAVEFSHRSGRDLINLAKKRTNI
  IPVIEDARHPHKYRMLIAMVDVIFADVAQPDQTRIVALNAHTFLRNGGHFVISIKANCIDSTASAEAVFA
  SEVKKMQQENMKPQEQLTLEPYERDHAVVVGVYRPPPKVKN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_001427
• RefSeq Size: 1135
• RefSeq ORF: 963
• Synonyms: FIB; FLRN; Nop1; RNU3IP1
• Locus ID: 2091
• UniProt ID: P22087
• Cytogenetics: 19q13.2
• Summary: 'This gene product is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. The encoded protein contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Its central region resembles an RNA-binding domain and contains an RNP consensus sequence. Antisera from approximately 8% of humans with the autoimmune disease scleroderma recognize fibrillarin. [provided by RefSeq, Jul 2008]'
• Protein Families: Stem cell - Pluripotency