Dehydrodolichyl Diphosphate Synthase (DHDDS) (NM_024887) Human Mass Spec Standard

CAT#: PH301430

DHDDS MS Standard C13 and N15-labeled recombinant protein (NP_079163)

Product Images

Coomassie blue staining of purified DHDDS protein (Cat# TP301430). The protein was produced from HEK293T cells transfected with DHDDS cDNA clone (Cat# RC201430) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC201430
• Predicted MW: 38.7 kDa
• Protein Sequence:
  Sequence Link

  >RC201430 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSWIKEGELSLWERFCANIIKAGPMPKHIAFIMDGNRRYAKKCQVERQEGHSQGFNKLAETLRWCLNLGI
  LEVTVYAFSIENFKRSKSEVDGLMDLARQKFSRLMEEKEKLQKHGVCIRVLGDLHLLPLDLQELIAQAVQ
  ATKNYNKCFLNVCFAYTSRHEISNAVREMAWGVEQGLLDPSDISESLLDKCLYTNRSPHPDILIRTSGEV
  RLSDFLLWQTSHSCLVFQPVLWPEYTFWNLFEAILQFQMNHSMLQKARDMYAEERKRQQLERDQATVTEQ
  LLREGLQASGDAQLRRTRLHKLSARREERVQGFLQALELKRADWLARLGTASA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_079163
• RefSeq Size: 3343
• RefSeq ORF: 909
• Synonyms: CIT; CPT; DEDSM; DS; hCIT; HDS; RP59
• Locus ID: 79947
• UniProt ID: Q86SQ9
• Cytogenetics: 1p36.11
• Summary: The protein encoded by this gene catalyzes cis-prenyl chain elongation to produce the polyprenyl backbone of dolichol, a glycosyl carrier lipid required for the biosynthesis of several classes of glycoproteins. Mutations in this gene are associated with retinitis pigmentosa type 59. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011]
• Protein Pathways: Terpenoid backbone biosynthesis