Dehydrodolichyl Diphosphate Synthase (DHDDS) (NM_024887) Human Recombinant Protein

CAT#: TP301430

Recombinant protein of human dehydrodolichyl diphosphate synthase (DHDDS), transcript variant 1

Product Images

Coomassie blue staining of purified DHDDS protein (Cat# TP301430). The protein was produced from HEK293T cells transfected with DHDDS cDNA clone (Cat# RC201430) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Species: Human
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  >RC201430 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSWIKEGELSLWERFCANIIKAGPMPKHIAFIMDGNRRYAKKCQVERQEGHSQGFNKLAETLRWCLNLGI
  LEVTVYAFSIENFKRSKSEVDGLMDLARQKFSRLMEEKEKLQKHGVCIRVLGDLHLLPLDLQELIAQAVQ
  ATKNYNKCFLNVCFAYTSRHEISNAVREMAWGVEQGLLDPSDISESLLDKCLYTNRSPHPDILIRTSGEV
  RLSDFLLWQTSHSCLVFQPVLWPEYTFWNLFEAILQFQMNHSMLQKARDMYAEERKRQQLERDQATVTEQ
  LLREGLQASGDAQLRRTRLHKLSARREERVQGFLQALELKRADWLARLGTASA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Tag: C-Myc/DDK
• Predicted MW: 38.6 kDa
• Concentration: >50 ug/mL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Note: For culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_079163
• Locus ID: 79947
• UniProt ID: Q86SQ9
• Cytogenetics: 1p36.11
• Refseq Size: 3343
• Refseq ORF: 909
• Synonyms: CIT; CPT; DEDSM; DS; hCIT; HDS; RP59
• Summary: The protein encoded by this gene catalyzes cis-prenyl chain elongation to produce the polyprenyl backbone of dolichol, a glycosyl carrier lipid required for the biosynthesis of several classes of glycoproteins. Mutations in this gene are associated with retinitis pigmentosa type 59. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011]
• Protein Pathways: Terpenoid backbone biosynthesis