PGP9.5 (UCHL1) (NM_004181) Human Mass Spec Standard

CAT#: PH301803

UCHL1 MS Standard C13 and N15-labeled recombinant protein (NP_004172)

Product Images

Coomassie blue staining of purified UCHL1 protein (Cat# TP301803). The protein was produced from HEK293T cells transfected with UCHL1 cDNA clone (Cat# RC201803) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC201803
• Predicted MW: 24.8 kDa
• Protein Sequence:
  Sequence Link

  >RC201803 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MQLKPMEINPEMLNKVLSRLGVAGQWRFVDVLGLEEESLGSVPAPACALLLLFPLTAQHENFRKKQIEEL
  KGQEVSPKVYFMKQTIGNSCGTIGLIHAVANNQDKLGFEDGSVLKQFLSETEKMSPEDRAKCFEKNEAIQ
  AAHDAVAQEGQCRVDDKVNFHFILFNNVDGHLYELDGRMPFPVNHGASSEDTLLKDAAKVCREFTEREQG
  EVRFSAVALCKAA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_004172
• RefSeq Size: 1141
• RefSeq ORF: 669
• Synonyms: HEL-117; HEL-S-53; NDGOA; PARK5; PGP 9.5; PGP9.5; PGP95; SPG79; Uch-L1
• Locus ID: 7345
• UniProt ID: P09936, V9HW74
• Cytogenetics: 4p13
• Summary: 'The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease.[provided by RefSeq, Sep 2009]'
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Parkinson's disease