EAP30 (SNF8) (NM_007241) Human Mass Spec Standard

CAT#: PH302137

SNF8 MS Standard C13 and N15-labeled recombinant protein (NP_009172)

Product Images

Coomassie blue staining of purified SNF8 protein (Cat# TP302137). The protein was produced from HEK293T cells transfected with SNF8 cDNA clone (Cat# RC202137) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC202137
• Predicted MW: 28.9 kDa
• Protein Sequence:
  Sequence Link

  >RC202137 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MHRRGVGAGAIAKKKLAEAKYKERGTVLAEDQLAQMSKQLDMFKTNLEEFASKHKQEIRKNPEFRVQFQD
  MCATIGVDPLASGKGFWSEMLGVGDFYYELGVQIIEVCLALKHRNGGLITLEELHQQVLKGRGKFAQDVS
  QDDLIRAIKKLKALGTGFGIIPVGGTYLIQSVPAELNMDHTVVLQLAEKNGYVTVSEIKASLKWETERAR
  QVLEHLLKEGLAWLDLQAPGEAHYWLPALFTDLYSQEITAEEAREALP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_009172
• RefSeq Size: 1226
• RefSeq ORF: 774
• Synonyms: Dot3; EAP30; VPS22
• Locus ID: 11267
• UniProt ID: Q96H20
• Cytogenetics: 17q21.32
• Summary: The protein encoded by this gene is a component of the endosomal sorting complex required for transport II (ESCRT-II), which regulates the movement of ubiquitinylated transmembrane proteins to the lysosome for degradation. This complex also interacts with the RNA polymerase II elongation factor (ELL) to overcome the repressive effects of ELL on RNA polymerase II activity. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015]
• Protein Families: Transcription Factors
• Protein Pathways: Endocytosis