UQCRB (NM_006294) Human Mass Spec Standard

CAT#: PH302685

UQCRB MS Standard C13 and N15-labeled recombinant protein (NP_006285)

Product Images

Coomassie blue staining of purified UQCRB protein (Cat# TP302685). The protein was produced from HEK293T cells transfected with UQCRB cDNA clone (Cat# RC202685) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC202685
• Predicted MW: 13.5 kDa
• Protein Sequence:
  Sequence Link

  >RC202685 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAGKQAVSASGKWLDGIRKWYYNAAGFNKLGLMRDDTIYEDEDVKEAIRRLPENLYNDRMFRIKRALDLN
  LKHQILPKEQWTKYEEENFYLEPYLKEVIRERKEREEWAKK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_006285
• RefSeq Size: 4839
• RefSeq ORF: 333
• Synonyms: MC3DN3; QCR7; QP-C; QPC; UQBC; UQBP; UQCR6; UQPC
• Locus ID: 7381
• UniProt ID: P14927
• Cytogenetics: 8q22.1
• Summary: 'This gene encodes a subunit of the ubiquinol-cytochrome c oxidoreductase complex, which consists of one mitochondrial-encoded and 10 nuclear-encoded subunits. The protein encoded by this gene binds ubiquinone and participates in the transfer of electrons when ubiquinone is bound. This protein plays an important role in hypoxia-induced angiogenesis through mitochondrial reactive oxygen species-mediated signaling. Mutations in this gene are associated with mitochondrial complex III deficiency. Alternatively spliced transcript variants have been found for this gene. Related pseudogenes have been identified on chromosomes 1, 5 and X. [provided by RefSeq, Dec 2011]'
• Protein Pathways: Alzheimer's disease, Cardiac muscle contraction, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease