NDUFA2 (NM_002488) Human Mass Spec Standard

CAT#: PH302715

NDUFA2 MS Standard C13 and N15-labeled recombinant protein (NP_002479)

Product Images

Coomassie blue staining of purified NDUFA2 protein (Cat# TP302715). The protein was produced from HEK293T cells transfected with NDUFA2 cDNA clone (Cat# RC202715) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC202715
• Predicted MW: 10.9 kDa
• Protein Sequence:
  Sequence Link

  >RC202715 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAAAASRGVGAKLGLREIRIHLCQRSPGSQGVRDFIEKRYVELKKANPDLPILIRECSDVQPKLWARYA
  FGQETNVPLNNFSADQVTRALENVLSGKA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_002479
• RefSeq Size: 726
• RefSeq ORF: 297
• Synonyms: B8; CD14; CIB8; MC1DN13
• Locus ID: 4695
• UniProt ID: O43678
• Cytogenetics: 5q31.3
• Summary: 'The encoded protein is a subunit of the hydrophobic protein fraction of the NADH:ubiquinone oxidoreductase (complex 1), the first enzyme complex in the electron transport chain located in the inner mitochondrial membrane, and may be involved in regulating complex I activity or its assembly via assistance in redox processes. Mutations in this gene are associated with Leigh syndrome, an early-onset progressive neurodegenerative disorder. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010]'
• Protein Pathways: Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease