COUP TF1 (NR2F1) (NM_005654) Human Mass Spec Standard

CAT#: PH303924

NR2F1 MS Standard C13 and N15-labeled recombinant protein (NP_005645)

Product Images

Coomassie blue staining of purified NR2F1 protein (Cat# TP303924). The protein was produced from HEK293T cells transfected with NR2F1 cDNA clone (Cat# RC203924) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC203924
• Predicted MW: 46.6 kDa
• Protein Sequence:
  Sequence Link

  >RC203924 representing NM_005654
  Red=Cloning site Green=Tags(s)
  
  MAMVVSSWRDPQDDVAGGNPGGPNPAAQAARGGGGGAGEQQQQAGSGAPHTPQTPGQPGAPATPGTAGDK
  GQGPPGSGQSQQHIECVVCGDKSSGKHYGQFTCEGCKSFFKRSVRRNLTYTCRANRNCPIDQHHRNQCQY
  CRLKKCLKVGMRREAVQRGRMPPTQPNPGQYALTNGDPLNGHCYLSGYISLLLRAEPYPTSRYGSQCMQP
  NNIMGIENICELAARLLFSAVEWARNIPFFPDLQITDQVSLLRLTWSELFVLNAAQCSMPLHVAPLLAAA
  GLHASPMSADRVVAFMDHIRIFQEQVEKLKALHVDSAEYSCLKAIVLFTSDACGLSDAAHIESLQEKSQC
  ALEEYVRSQYPNQPSRFGKLLLRLPSLRTVSSSVIEQLFFVRLVGKTPIETLIRDMLLSGSSFNWPYMSI
  QCS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_005645
• RefSeq Size: 3210
• RefSeq ORF: 1269
• Synonyms: BBOAS; BBSOAS; COUP-TFI; COUPTF1; EAR-3; EAR3; ERBAL3; SVP44; TCFCOUP1; TFCOUP1
• Locus ID: 7025
• UniProt ID: P10589
• Cytogenetics: 5q15
• Summary: 'The protein encoded by this gene is a nuclear hormone receptor and transcriptional regulator. The encoded protein acts as a homodimer and binds to 5'-AGGTCA-3' repeats. Defects in this gene are a cause of Bosch-Boonstra optic atrophy syndrome (BBOAS). [provided by RefSeq, Apr 2014]'
• Protein Families: Druggable Genome, Nuclear Hormone Receptor, Transcription Factors