MRPL43 (NM_032112) Human Mass Spec Standard
CAT#: PH303936
MRPL43 MS Standard C13 and N15-labeled recombinant protein (NP_115488)
Specifications
Product Data | |
Tag | C-Myc/DDK |
Species | Human |
Expression Host | HEK293 |
Expression cDNA Clone or AA Sequence | RC203936 |
Predicted MW | 17.9 kDa |
Protein Sequence |
>RC203936 protein sequence
Red=Cloning site Green=Tags(s) MTARGTPSRFLASVLHNGLGRYVQQLQRLSFSVSRDGASSRGAREFVEREVIDFARRNPGVVIYVNSRPC CVPRVVAEYLNGAVREESIHCKSVEEISTLVQKLADQSGLDVIRIRKPFHTDNPSIQGQWHPFTNKPTTF RGLRPREVQDPAPAQVQAQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
Concentration | 50 ug/ml as determined by BCA |
Labeling Method | Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine |
Buffer | 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions. |
Reference Data | |
RefSeq | NP_115488 |
RefSeq Size | 1016 |
RefSeq ORF | 477 |
Synonyms | bMRP36a; L43mt; MRP-L43 |
Locus ID | 84545 |
UniProt ID | Q8N983, A8K4V4 |
Cytogenetics | 10q24.31 |
Summary | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. This gene and the gene for a semaphorin class 4 protein (SEMA4G) overlap at map location 10q24.31 and are transcribed in opposite directions. Sequence analysis identified multiple transcript variants encoding at least four different protein isoforms. [provided by RefSeq, Jul 2008] |
Documents
FAQs |
Resources
Recombinant Protein Resources |
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
LC406120 | MRPL43 HEK293T cell transient overexpression lysate (as WB positive control) |
USD 121.00 |
|
LC406122 | MRPL43 HEK293T cell transient overexpression lysate (as WB positive control) |
USD 121.00 |
|
LC410343 | MRPL43 HEK293T cell transient overexpression lysate (as WB positive control) |
USD 121.00 |
|
LY406120 | Transient overexpression lysate of mitochondrial ribosomal protein L43 (MRPL43), nuclear gene encoding mitochondrial protein, transcript variant 2 |
USD 396.00 |
|
LY406122 | Transient overexpression lysate of mitochondrial ribosomal protein L43 (MRPL43), nuclear gene encoding mitochondrial protein, transcript variant 4 |
USD 396.00 |
|
LY410343 | Transient overexpression lysate of mitochondrial ribosomal protein L43 (MRPL43), nuclear gene encoding mitochondrial protein, transcript variant 1 |
USD 396.00 |
|
PH308068 | MRPL43 MS Standard C13 and N15-labeled recombinant protein (NP_789762) |
USD 2,055.00 |
|
TP303936 | Recombinant protein of human mitochondrial ribosomal protein L43 (MRPL43), nuclear gene encoding mitochondrial protein, transcript variant 1 |
USD 823.00 |
|
TP308068 | Recombinant protein of human mitochondrial ribosomal protein L43 (MRPL43), nuclear gene encoding mitochondrial protein, transcript variant 2 |
USD 823.00 |
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