Rad9 (RAD9A) (NM_004584) Human Mass Spec Standard

CAT#: PH304439

RAD9A MS Standard C13 and N15-labeled recombinant protein (NP_004575)

Product Images

Coomassie blue staining of purified RAD9A protein (Cat# TP304439). The protein was produced from HEK293T cells transfected with RAD9A cDNA clone (Cat# RC204439) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC204439
• Predicted MW: 42.5 kDa
• Protein Sequence:
  Sequence Link

  >RC204439 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKCLVTGGNVKVLGKAVHSLSRIGDELYLEPLEDGLSLRTVNSSRSAYACFLFAPLFFQQYQAATPGQDL
  LRCKILMKSFLSVFRSLAMLEKTVEKCCISLNGRSSRLVVQLHCKFGVRKTHNLSFQDCASLQAVFDPAS
  CPHMLRAPARVLGEAVLPFSPALAEVTLGIGRGRRVILRSYHEEEADSTAKAMVTEMCLGEEDFQQLQAQ
  EGVAITFCLKEFRGLLSFAESANLNLSIHFDAPGRPAIFTIKDSLLDGHFVLATLSDTDSHSQDLGSPER
  HQPVPQLQAHSTPHPDDFANDDIDSYMIAMETTIGNEGSRVLPSISLSPGPQPPKSPGPHSEEEDEAEPS
  TVPGTPPPKKFRSLFFGSILAPVRSPQGPSPVLAEDSEGEG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_004575
• RefSeq Size: 2128
• RefSeq ORF: 1173
• Synonyms: RAD9
• Locus ID: 5883
• UniProt ID: Q99638
• Cytogenetics: 11q13.2
• Summary: 'This gene product is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair. This protein possesses 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]'
• Protein Families: Druggable Genome, Stem cell - Pluripotency