Endothelin B Receptor (EDNRB) (NM_000115) Human Mass Spec Standard

CAT#: PH304899

EDNRB MS Standard C13 and N15-labeled recombinant protein (NP_000106)

Product Images

Coomassie blue staining of purified EDNRB protein (Cat# TP304899). The protein was produced from HEK293T cells transfected with EDNRB cDNA clone (Cat# RC204899) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC204899
• Predicted MW: 49.6 kDa
• Protein Sequence:
  Sequence Link

  >RC204899 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MQPPPSLCGRALVALVLACGLSRIWGEERGFPPDRATPLLQTAEIMTPPTKTLWPKGSNASLARSLAPAE
  VPKGDRTAGSPPRTISPPPCQGPIEIKETFKYINTVVSCLVFVLGIIGNSTLLRIIYKNKCMRNGPNILI
  ASLALGDLLHIVIDIPINVYKLLAEDWPFGAEMCKLVPFIQKASVGITVLSLCALSIDRYRAVASWSRIK
  GIGVPKWTAVEIVLIWVVSVVLAVPEAIGFDIITMDYKGSYLRICLLHPVQKTAFMQFYKTAKDWWLFSF
  YFCLPLAITAFFYTLMTCEMLRKKSGMQIALNDHLKQRREVAKTVFCLVLVFALCWLPLHLSRILKLTLY
  NQNDPNRCELLSFLLVLDYIGINMASLNSCINPIALYLVSKRFKNCFKSCLCCWCQSFEEKQSLEEKQSC
  LKFKANDHGYDNFRSSNKYSSS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_000106
• RefSeq Size: 4296
• RefSeq ORF: 1326
• Synonyms: ABCDS; ET-B; ET-BR; ETB; ETB1; ETBR; ETRB; HSCR; HSCR2; WS4A
• Locus ID: 1910
• UniProt ID: P24530
• Cytogenetics: 13q22.3
• Summary: 'The protein encoded by this gene is a G protein-coupled receptor which activates a phosphatidylinositol-calcium second messenger system. Its ligand, endothelin, consists of a family of three potent vasoactive peptides: ET1, ET2, and ET3. Studies suggest that the multigenic disorder, Hirschsprung disease type 2, is due to mutations in the endothelin receptor type B gene. Alternative splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, Oct 2016]'
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Calcium signaling pathway, Melanogenesis, Neuroactive ligand-receptor interaction