ARF4 (NM_001660) Human Mass Spec Standard

CAT#: PH304984

ARF4 MS Standard C13 and N15-labeled recombinant protein (NP_001651)

Product Images

Coomassie blue staining of purified ARF4 protein (Cat# TP304984). The protein was produced from HEK293T cells transfected with ARF4 cDNA clone (Cat# RC204984) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC204984
• Predicted MW: 20.5 kDa
• Protein Sequence:
  Sequence Link

  >RC204984 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGLTISSLFSRLFGKKQMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNICFTVWDVGG
  QDRIRPLWKHYFQNTQGLIFVVDSNDRERIQEVADELQKMLLVDELRDAVLLLFANKQDLPNAMAISEMT
  DKLGLQSLRNRTWYVQATCATQGTGLYEGLDWLSNELSKR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_001651
• RefSeq Size: 1758
• RefSeq ORF: 540
• Synonyms: ARF2
• Locus ID: 378
• UniProt ID: P18085
• Cytogenetics: 3p14.3
• Summary: This gene is a member of the human ARF gene family whose members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 5 ARF proteins and 11 ARF-like proteins and constitute one family of the RAS superfamily. The ARF proteins are categorized as class I, class II and class III; this gene is a class II member. The members of each class share a common gene organization. The ARF4 gene spans approximately 12kb and contains six exons and five introns. This gene is the most divergent member of the human ARFs. Conflicting map positions at 3p14 or 3p21 have been reported for this gene. [provided by RefSeq, Jul 2008]