CYB5R4 (NM_016230) Human Mass Spec Standard

CAT#: PH305203

CYB5R4 MS Standard C13 and N15-labeled recombinant protein (NP_057314)

Product Images

Coomassie blue staining of purified CYB5R4 protein (Cat# TP305203). The protein was produced from HEK293T cells transfected with CYB5R4 cDNA clone (Cat# RC205203) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC205203
• Predicted MW: 59.5 kDa
• Protein Sequence:
  Sequence Link

  >RC205203 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLNVPSQSFPAPRSQQRVASGGRSKVPLKQGRSLMDWIRLTKSGKDLTGLKGRLIEVTEEELKKHNKKDD
  CWICIRGFVYNVSPYMEYHPGGEDELMRAAGSDGTELFDQVHRWVNYESMLKECLVGRMAIKPAVLKDYR
  EEEKKVLNGMLPKSQVTDTLAKEGPSYPSYDWFQTDSLVTIAIYTKQKDINLDSIIVDHQNDSFRAETII
  KDCLYLIHIGLSHEVQEDFSVRVVESVGKIEIVLQKKENTSWDFLGHPLKNHNSLIPRKDTGLYYRKCQL
  ISKEDVTHDTRLFCLMLPPSTHLQVPIGQHVYLKLPITGTEIVKPYTPVSGSLLSEFKEPVLPNNKYIYF
  LIKIYPTGLFTPELDRLQIGDFVSVSSPEGNFKISKFQELEDLFLLAAGTGFTPMVKILNYALTDIPSLR
  KVKLMFFNKTEDDIIWRSQLEKLAFKDKRLDVEFVLSAPISEWNGKQGHISPALLSEFLKRNLDKSKVLV
  CICGPVPFTEQGVRLLHDLNFSKNEIHSFTA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_057314
• RefSeq Size: 2259
• RefSeq ORF: 1563
• Synonyms: cb5/cb5R; dJ676J13.1; NCB5OR
• Locus ID: 51167
• UniProt ID: Q7L1T6
• Cytogenetics: 6q14.2
• Summary: NCB5OR is a flavohemoprotein that contains functional domains found in both cytochrome b5 (CYB5A; MIM 613218) and CYB5 reductase (CYB5R3; MIM 613213) (Zhu et al., 1999 [PubMed 10611283]). [supplied by OMIM, Jan 2010]
• Protein Families: Druggable Genome