VPS26 (VPS26A) (NM_004896) Human Mass Spec Standard

CAT#: PH305353

VPS26A MS Standard C13 and N15-labeled recombinant protein (NP_004887)

Product Images

Coomassie blue staining of purified VPS26A protein (Cat# TP305353). The protein was produced from HEK293T cells transfected with VPS26A cDNA clone (Cat# RC205353) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC205353
• Predicted MW: 38.2 kDa
• Protein Sequence:
  Sequence Link

  >RC205353 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSFLGGFFGPICEIDIVLNDGETRKMAEMKTEDGKVEKHYLFYDGESVSGKVNLAFKQPGKRLEHQGIRI
  EFVGQIELFNDKSNTHEFVNLVKELALPGELTQSRSYDFEFMQVEKPYESYIGANVRLRYFLKVTIVRRL
  TDLVKEYDLIVHQLATYPDVNNSIKMEVGIEDCLHIEFEYNKSKYHLKDVIVGKIYFLLVRIKIQHMELQ
  LIKKEITGIGPSTTTETETIAKYEIMDGAPVKGESIPIRLFLAGYDPTPTMRDVNKKFSVRYFLNLVLVD
  EEDRRYFKQQEIILWRKAPEKLRKQRTNFHQRFESPESQASAEQPEM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_004887
• RefSeq Size: 2707
• RefSeq ORF: 981
• Synonyms: HB58; Hbeta58; PEP8A; VPS26
• Locus ID: 9559
• UniProt ID: O75436
• Cytogenetics: 10q22.1
• Summary: This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]