MARCH8 (NM_001002266) Human Mass Spec Standard

CAT#: PH305477

MARCH8 MS Standard C13 and N15-labeled recombinant protein (NP_001002266)

Product Images

Coomassie blue staining of purified MARCH8 protein (Cat# TP305477). The protein was produced from HEK293T cells transfected with MARCH8 cDNA clone (Cat# RC205477) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC205477
• Predicted MW: 32.9 kDa
• Protein Sequence:
  Sequence Link

  >RC205477 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSMPLHQISAIPSQDAISARVYRSKTKEKEREEQNEKTLGHFMSHSSNISKAGSPPSASAPAPVSSFSRT
  SITPSSQDICRICHCEGDDESPLITPCHCTGSLHFVHQACLQQWIKSSDTRCCELCKYEFIMETKLKPLR
  KWEKLQMTSSERRKIMCSVTFHVIAITCVVWSLYVLIDRTAEEIKQGQATGILEWPFWTKLVVVAIGFTG
  GLLFMYVQCKVYVQLWKRLKAYNRVIYVQNCPETSKKNIFEKSPLTEPNFENKHGHGICHSDTNSSCCTE
  PEDTGAEIIHV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_001002266
• RefSeq Size: 4812
• RefSeq ORF: 873
• Synonyms: c-MIR; CMIR; MARCH-VIII; MARCH8; MIR; RNF178
• Locus ID: 220972
• UniProt ID: Q5T0T0, A0A024R7U2
• Cytogenetics: 10q11.21-q11.22
• Summary: MARCH8 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments. MARCH8 induces the internalization of several membrane glycoproteins (Goto et al., 2003 [PubMed 12582153]; Bartee et al., 2004 [PubMed 14722266]). [supplied by OMIM, Apr 2010]
• Protein Families: Druggable Genome, Transmembrane