DEPTOR (NM_022783) Human Mass Spec Standard

CAT#: PH305770

DEPDC6 MS Standard C13 and N15-labeled recombinant protein (NP_073620)

Product Images

Coomassie blue staining of purified DEPTOR protein (Cat# TP305770). The protein was produced from HEK293T cells transfected with DEPTOR cDNA clone (Cat# RC205770) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC205770
• Predicted MW: 46.3 kDa
• Protein Sequence:
  Sequence Link

  >RC205770 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEEGGSTGSAGSDSSTSGSGGAQQRELERMAEVLVTGEQLRLRLHEEKVIKDRRHHLKTYPNCFVAKELI
  DWLIEHKEASDRETAIKLMQKLADRGIIHHVCDEHKEFKDVKLFYRFRKDDGTFPLDNEVKAFMRGQRLY
  EKLMSPENTLLQPREEEGVKYERTFMASEFLDWLVQEGEATTRKEAEQLCHRLMEHGIIQHVSSKHPFVD
  SNLLYQFRMNFRRRRRLMELLNEKSPSSQETHDSPFCLRKQSHDNRKSTSFMSVSPSKEIKIVSAVRRSS
  MSSCGSSGYFSSSPTLSSSPPVLCNPKSVLKRPVTSEELLTPGAPYARKTFTIVGDAVGWGFVVRGSKPC
  HIQAVDPSGPAAAAGMKVCQFVVSVNGLNVLHVDYRTVSNLILTGPRTIVMEVMEELEC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_073620
• RefSeq Size: 2636
• RefSeq ORF: 1227
• Synonyms: DEP.6; DEPDC6
• Locus ID: 64798
• UniProt ID: Q8TB45
• Cytogenetics: 8q24.12