CSHL1 (NM_001318) Human Mass Spec Standard

CAT#: PH305897

CSHL1 MS Standard C13 and N15-labeled recombinant protein (NP_001309)

Product Images

Coomassie blue staining of purified CSHL1 protein (Cat# TP305897). The protein was produced from HEK293T cells transfected with CSHL1 cDNA clone (Cat# RC205897) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC205897
• Predicted MW: 14.9 kDa
• Protein Sequence:
  Sequence Link

  >RC205897 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEETQQKSNLELLHISLLLIESRLEPVRFLRSTFTNNLVYDTSDSDDYHLLKDLEEGIQMLMGRLEDGSH
  LTGQTLKQTYSKFDTNSHNHDALLKNYGLLHCFRKDMDKVETFLRMVQCRSVEGSCGF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_001309
• RefSeq Size: 661
• RefSeq ORF: 384
• Synonyms: CS-5; CSHP1; CSL; GHB4; hCS-L
• Locus ID: 1444
• UniProt ID: I6L999
• Cytogenetics: 17q23.3
• Summary: 'The protein encoded by this gene is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. The gene, along with four other related genes, is located at the growth hormone locus on chromosome 17 where they are interspersed in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. Although the five genes share a remarkably high degree of sequence identity, they are expressed selectively in different tissues. This particular family member is expressed in placental villi, although it was originally thought to be a pseudogene. In fact, alternative splicing suggests that the majority of the transcripts would be unable to express a secreted protein. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]'
• Protein Families: Secreted Protein