BIRC5 (NM_001168) Human Mass Spec Standard

CAT#: PH305935

BIRC5/Survivin MS Standard C13 and N15-labeled recombinant protein (NP_001159)

Product Images

Coomassie blue staining of purified BIRC5 protein (Cat# TP305935). The protein was produced from HEK293T cells transfected with BIRC5 cDNA clone (Cat# RC205935) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC205935
• Predicted MW: 16.2 kDa
• Protein Sequence:
  Sequence Link

  >RC205935 representing NM_001168
  Red=Cloning site Green=Tags(s)
  
  MGAPTLPPAWQPFLKDHRISTFKNWPFLEGCACTPERMAEAGFIHCPTENEPDLAQCFFCFKELEGWEPD
  DDPIEEHKKHSSGCAFLSVKKQFEELTLGEFLKLDRERAKNKIAKETNNKKKEFEETAKKVRRAIEQLAA
  MD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_001159
• RefSeq Size: 2655
• RefSeq ORF: 426
• Synonyms: API4; EPR-1
• Locus ID: 332
• UniProt ID: O15392, A0A0B4J1S3
• Cytogenetics: 17q25.3
• Summary: 'This gene is a member of the inhibitor of apoptosis (IAP) gene family, which encode negative regulatory proteins that prevent apoptotic cell death. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but this gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors, yet low in adult tissues. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jun 2011]'
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Colorectal cancer, Pathways in cancer