ZFYVE27 (NM_144588) Human Mass Spec Standard

CAT#: PH306193

ZFYVE27 MS Standard C13 and N15-labeled recombinant protein (NP_653189)

Product Images

Coomassie blue staining of purified ZFYVE27 protein (Cat# TP306193). The protein was produced from HEK293T cells transfected with ZFYVE27 cDNA clone (Cat# RC206193) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC206193
• Predicted MW: 45.8 kDa
• Protein Sequence:
  Sequence Link

  >RC206193 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MQTSEREGSGPELSPSVMPEAPLESPPFPTKSPAFDLFNLVLSYKRLEINLEPLKDAGDGVRYLLRWQMP
  LCSLLTCLGLNVLFLTLNEGAWYSVGALMISVPALLGYLQEVCRARLPDSELMRRKYHSVRQEDLQRVRL
  SRPEAVAEVKSFLIQLEAFLSRLCCTCEAAYRVLHWENPVVSSQFYGALLGTVCMLYLLPLCWVLTLLNS
  TLFLGNVEFFRVVSEYRASLQQRMNPKQEEHAFESPPPPDVGGKDGLMDSTPALTPTEDLTPGSVEEAEE
  AEPDEEFKDAIEETHLVVLEDDEGAPCPAEDELALQDNGFLSKNEVLRSKVSRLTERLRKRYPTNNFGNC
  TGCSATFSVLKKRRSCSNCGNSFCSRCCSFKVPKSSMGATAPEAQRETVFVCASCNQTLSK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_653189
• RefSeq Size: 3059
• RefSeq ORF: 1233
• Synonyms: PROTRUDIN; SPG33
• Locus ID: 118813
• UniProt ID: Q5T4F4
• Cytogenetics: 10q24.2
• Summary: This gene encodes a protein with several transmembrane domains, a Rab11-binding domain and a lipid-binding FYVE finger domain. The encoded protein appears to promote neurite formation. A mutation in this gene has been reported to be associated with hereditary spastic paraplegia, however the pathogenicity of the mutation, which may simply represent a polymorphism, is unclear. [provided by RefSeq, Mar 2010]
• Protein Families: Transmembrane