Estrogen Sulfotransferase (SULT1E1) (NM_005420) Human Mass Spec Standard

CAT#: PH306535

SULT1E1 MS Standard C13 and N15-labeled recombinant protein (NP_005411)

Product Images

Coomassie blue staining of purified SULT1E1 protein (Cat# TP306535). The protein was produced from HEK293T cells transfected with SULT1E1 cDNA clone (Cat# RC206535) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC206535
• Predicted MW: 35.1 kDa
• Protein Sequence:
  Sequence Link

  >RC206535 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNSELDYYEKFEEVHGILMYKDFVKYWDNVEAFQARPDDLVIATYPKSGTTWVSEIVYMIYKEGDVEKCK
  EDVIFNRIPFLECRKENLMNGVKQLDEMNSPRIVKTHLPPELLPASFWEKDCKIIYLCRNAKDVAVSFYY
  FFLMVAGHPNPGSLPEFVEKFMQGQVPYGSWYKHVKSWWEKGKSPRVLFLFYEDLKEDIRKEVIKLIHFL
  ERKPSEELVDRIIHHTSFQEMKNNPSTNYTTLPDEIMNQKLSPFMRKGITGDWKNHFTVALNEKFDKHYE
  QQMKESTLKFRTEI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_005411
• RefSeq Size: 1805
• RefSeq ORF: 882
• Synonyms: EST; EST-1; ST1E1; STE
• Locus ID: 6783
• UniProt ID: P49888, Q53X91
• Cytogenetics: 4q13.3
• Summary: 'Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and substrate specificities. The gene structure (number and length of exons) is similar among family members. This gene encodes a protein that transfers a sulfo moiety to and from estrone, which may control levels of estrogen receptors. [provided by RefSeq, Jul 2008]'
• Protein Pathways: Androgen and estrogen metabolism, Sulfur metabolism