E3 SUMO protein ligase PIAS4 (PIAS4) (NM_015897) Human Mass Spec Standard

CAT#: PH306748

PIAS4 MS Standard C13 and N15-labeled recombinant protein (NP_056981)

Product Images

Coomassie blue staining of purified PIAS4 protein (Cat# TP306748). The protein was produced from HEK293T cells transfected with PIAS4 cDNA clone (Cat# RC206748) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC206748
• Predicted MW: 56.5 kDa
• Protein Sequence:
  Sequence Link

  >RC206748 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAELVEAKNMVMSFRVSDLQMLLGFVGRSKSGLKHELVTRALQLVQFDCSPELFKKIKELYETRYAKKN
  SEPAPQPHRPLDPLTMHSTYDRAGAVPRTPLAGPNIDYPVLYGKYLNGLGRLPAKTLKPEVRLVKLPFFN
  MLDELLKPTELVPQNNEKLQESPCIFALTPRQVELIRNSRELQPGVKAVQVVLRICYSDTSCPQEDQYPP
  NIAVKVNHSYCSVPGYYPSNKPGVEPKRPCRPINLTHLMYLSSATNRITVTWGNYGKSYSVALYLVRQLT
  SSELLQRLKTIGVKHPELCKALVKEKLRLDPDSEIATTGVRVSLICPLVKMRLSVPCRAETCAHLQCFDA
  VFYLQMNEKKPTWMCPVCDKPAPYDQLIIDGLLSKILSECEDADEIEYLVDGSWCPIRAEKERSCSPQGA
  ILVLGPSDANGLLPAPSVNGSGALGSTGGGGPVGSMENGKPGADVVDLTLDSSSSSEDEEEEEEEEEDED
  EEGPRPKRRCPFQKGLVPAC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_056981
• RefSeq Size: 1835
• RefSeq ORF: 1530
• Synonyms: PIAS-gamma; Piasg; PIASY; ZMIZ6
• Locus ID: 51588
• UniProt ID: Q8N2W9, B3KMR4
• Cytogenetics: 19p13.3
• Protein Families: Transcription Factors
• Protein Pathways: Jak-STAT signaling pathway, Pathways in cancer, Small cell lung cancer, Ubiquitin mediated proteolysis