MAPK4 (NM_002747) Human Mass Spec Standard

CAT#: PH307414

MAPK4 MS Standard C13 and N15-labeled recombinant protein (NP_002738)

Product Images

Coomassie blue staining of purified MAPK4 protein (Cat# TP307414). The protein was produced from HEK293T cells transfected with MAPK4 cDNA clone (Cat# RC207414) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC207414
• Predicted MW: 66 kDa
• Protein Sequence:
  Sequence Link

  >RC207414 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAEKGDCIASVYGYDLGGRFVDFQPLGFGVNGLVLSAMDSRACRKVAVKKIALSDARSMKHALREIKIIR
  RLDHDNIVKVYEVLGPKGTDLQGELFKFSVAYIVQEYMETDLARLLEQGTLAEEHAKLFMYQLLRGLKYI
  HSANVLHRDLKPANIFISTEDLVLKIGDFGLARIVDQHYSHKGYLSEGLVTKWYRSPRLLLSPNNYTKAI
  DMWAAGCILSEMLTGRMLFAGAHELEQMQLILETIPVIREEDKDELLRVMPSFVSSTWEVKRPLRKLLPE
  VNSEAIDFLEKILTFNPMDRLTAEMGLQHPYMSPYSCPEDEPTSQHPFRIEDEIDDIVLMAANQSQLSNW
  DTCSSRYPVSLSSDLEWRPDRCQDASEVQRDPRAGSAPLAEDVQVDPRKDSHSSSERFLEQSHSSMERAF
  EADYGRSCDYKVGSPSYLDKLLWRDNKPHHYSEPKLILDLSHWKQAAGAPPTATGLADTGAREDEPASLF
  LEIAQWVKSTQGGPEHASPPADDPERRLSASPPGRPAPVDGGASPQFDLDVFISRALKLCTKPEDLPDNK
  LGDLNGACIPEHPGDLVQTEAFSKERW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_002738
• RefSeq Size: 4736
• RefSeq ORF: 1761
• Synonyms: ERK-4; ERK4; p63-MAPK; p63MAPK; PRKM4
• Locus ID: 5596
• UniProt ID: P31152
• Cytogenetics: 18q21.1-q21.2
• Summary: 'Mitogen-activated protein kinase 4 is a member of the mitogen-activated protein kinase family. Tyrosine kinase growth factor receptors activate mitogen-activated protein kinases which then translocate into the nucleus and phosphorylate nuclear targets. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]'
• Protein Families: Druggable Genome, Protein Kinase