MT (MCAT) (NM_173467) Human Mass Spec Standard

CAT#: PH307943

MCAT MS Standard C13 and N15-labeled recombinant protein (NP_775738)

Product Images

Coomassie blue staining of purified MCAT protein (Cat# TP307943). The protein was produced from HEK293T cells transfected with MCAT cDNA clone (Cat# RC207943) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC207943
• Predicted MW: 42.9 kDa
• Protein Sequence:
  Sequence Link

  >RC207943 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSVRVARVAWVRGLGASYRRGASSFPVPPPGAQGVAELLRDATGAEEEAPWAATERRMPGQCSVLLFPGQ
  GSQVVGMGRGLLNYPRVRELYAAARRVLGYDLLELSLHGPQETLDRTVHCQPAIFVASLAAVEKLHHLQP
  SVIENCVAAAGFSVGEFAALVFAGAMEFAEGLYAVKIRAEAMQEASEAVPSGMLSVLGQPQSKFNFACLE
  AREHCKSLGIENPVCEVSNYLFPDCRVISGHQEALRFLQKNSSKFHFRRTRMLPVSGAFHTRLMEPAVEP
  LTQALKAVDIKKPLVSVYSNVHGHRYRHPGHIHKLLAQQLVSPVKWEQTMHAIYERKKGRGFPQTFEVGP
  GRQLGAILKSCNMQAWKSYSAVDVLQTLEHVDLDPQEPPR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_775738
• RefSeq Size: 2086
• RefSeq ORF: 1170
• Synonyms: fabD; FASN2C; MCT; MCT1; MT; NET62
• Locus ID: 27349
• UniProt ID: Q8IVS2
• Cytogenetics: 22q13.2
• Summary: The protein encoded by this gene is found exclusively in the mitochondrion, where it catalyzes the transfer of a malonyl group from malonyl-CoA to the mitochondrial acyl carrier protein. The encoded protein may be part of a fatty acid synthase complex that is more like the type II prokaryotic and plastid complexes rather than the type I human cytosolic complex. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2012]
• Protein Pathways: Fatty acid biosynthesis, Metabolic pathways