Synaptotagmin V (SYT5) (NM_003180) Human Mass Spec Standard

CAT#: PH308528

SYT5 MS Standard C13 and N15-labeled recombinant protein (NP_003171)

Product Images

Coomassie blue staining of purified SYT5 protein (Cat# TP308528). The protein was produced from HEK293T cells transfected with SYT5 cDNA clone (Cat# RC208528) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC208528
• Predicted MW: 42.9 kDa
• Protein Sequence:
  Sequence Link

  >RC208528 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MFPEPPTPGPPSPDTPPDSSRISHGPVPPWALATIVLVSGLLIFSCCFCLYRKSCRRRTGKKSQAQAQVH
  LQEVKGLGQSYIDKVQPEVEELEPAPSGPGQQVADKHELGQLQYSLDYDFQSGQLLVGILQAMGLAALDL
  GGSSDPYVRVYLLPDKRRRYETKVHRQTLNPHFGETFAFKVPYVELGGRVLVMAVYDFDRFSRNDAIGEV
  RVPMSSVDLGRPVQAWRELQAAPREEQEKLGDICFSLRYVPTAGKLTVIVLEAKNLKKMDVGGLSDPYVK
  VHLLQGGKKVRKKKTTIKKNTLNPYYNEAFSFEVPCDQVQKVQVELTVLDYDKLGKNEAIGRVAVGAAAG
  GAGLRHWADMLANPRRPIAQWHSLRPPDRVRLLPAP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_003171
• RefSeq Size: 1853
• RefSeq ORF: 1158
• Synonyms: synaptotagmin 5; synaptotagmin V
• Locus ID: 6861
• UniProt ID: O00445, A0A024R4N8
• Cytogenetics: 11p
• Summary: 'Synaptotagmins, such as SYT5, are a family of type III membrane proteins characterized by cytoplasmic repeats related to protein kinase C (see MIM 176960) regulatory (C2) domains, which are thought to bind calcium. Synaptotagmins may act both as negative regulators of vesicle fusion, allowing fusion in the presence of calcium, and as calcium receptors or sensor molecules (summary by Hudson and Birnbaum, 1995 [PubMed 7597049]).[supplied by OMIM, Feb 2011]'
• Protein Families: Secreted Protein, Transmembrane