UBE2L3 (NM_003347) Human Mass Spec Standard

CAT#: PH308780

UBE2L3 MS Standard C13 and N15-labeled recombinant protein (NP_003338)

Product Images

Coomassie blue staining of purified UBE2L3 protein (Cat# TP308780). The protein was produced from HEK293T cells transfected with UBE2L3 cDNA clone (Cat# RC208780) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC208780
• Predicted MW: 17.9 kDa
• Protein Sequence:
  Sequence Link

  >RC208780 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAASRRLMKELEEIRKCGMKNFRNIQVDEANLLTWQGLIVPDNPPYDKGAFRIEINFPAEYPFKPPKITF
  KTKIYHPNIDEKGQVCLPVISAENWKPATKTDQVIQSLIALVNDPQPEHPLRADLAEEYSKDRKKFCKNA
  EEFTKKYGEKRPVD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_003338
• RefSeq Size: 3028
• RefSeq ORF: 462
• Synonyms: E2-F1; L-UBC; UBCH7; UbcM4
• Locus ID: 7332
• UniProt ID: P68036, A0A024R1A4
• Cytogenetics: 22q11.21
• Summary: 'The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2009]'
• Protein Pathways: Parkinson's disease, Ubiquitin mediated proteolysis