MRPL54 (NM_172251) Human Mass Spec Standard
CAT#: PH308976
MRPL54 MS Standard C13 and N15-labeled recombinant protein (NP_758455)
Specifications
Product Data | |
Tag | C-Myc/DDK |
Species | Human |
Expression Host | HEK293 |
Expression cDNA Clone or AA Sequence | RC208976 |
Predicted MW | 15.8 kDa |
Protein Sequence |
>RC208976 protein sequence
Red=Cloning site Green=Tags(s) MATKRLFGATRTWAGWGAWELLNPATSGRLLARDYAKKPVMKGAKSGKGAVTSEALKDPDVCTDPVQLTT YAMGVNIYKEGQDVPLKPDAEYPEWLFEMNLGPPKTLEELDPESREYWRRLRKQNIWRHNRLSKNKRL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
Concentration | 50 ug/ml as determined by BCA |
Labeling Method | Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine |
Buffer | 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions. |
Reference Data | |
RefSeq | NP_758455 |
RefSeq Size | 628 |
RefSeq ORF | 414 |
Synonyms | L54mt; MRP-L54 |
Locus ID | 116541 |
UniProt ID | Q6P161 |
Cytogenetics | 19p13.3 |
Summary | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. [provided by RefSeq, Jul 2008] |
Documents
FAQs |
Resources
Recombinant Protein Resources |
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
LC406705 | MRPL54 HEK293T cell transient overexpression lysate (as WB positive control) |
USD 121.00 |
|
LY406705 | Transient overexpression lysate of mitochondrial ribosomal protein L54 (MRPL54), nuclear gene encoding mitochondrial protein |
USD 396.00 |
|
TP308976 | Recombinant protein of human mitochondrial ribosomal protein L54 (MRPL54), nuclear gene encoding mitochondrial protein |
USD 823.00 |
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