ORC6L (ORC6) (NM_014321) Human Mass Spec Standard

CAT#: PH309054

ORC6L MS Standard C13 and N15-labeled recombinant protein (NP_055136)

Product Images

Coomassie blue staining of purified ORC6 protein (Cat# TP309054). The protein was produced from HEK293T cells transfected with ORC6 cDNA clone (Cat# RC209054) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC209054
• Predicted MW: 28.1 kDa
• Protein Sequence:
  Sequence Link

  >RC209054 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGSELIGRLAPRLGLAEPDMLRKAEEYLRLSRVKCVGLSARTTETSSAVMCLDLAASWMKCPLDRAYLIK
  LSGLNKETYQSCLKSFECLLGLNSNIGIRDLAVQFSCIEAVNMASKILKSYESSLPQTQQVDLDLSRPLF
  TSAALLSACKILKLKVDKNKMVATSGVKKAIFDRLCKQLEKIGQQVDREPGDVATPPRKRKKIVVEAPAK
  EMEKVEEMPHKPQKDEDLTQDYEEWKRKILENAASAQKATAE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_055136
• RefSeq Size: 1644
• RefSeq ORF: 756
• Synonyms: ORC6L
• Locus ID: 23594
• UniProt ID: Q9Y5N6, A0A024R6R3
• Cytogenetics: 16q11.2
• Summary: The origin recognition complex (ORC) is a highly conserved six subunit protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is a subunit of the ORC complex. Gene silencing studies with small interfering RNA demonstrated that this protein plays an essential role in coordinating chromosome replication and segregation with cytokinesis. [provided by RefSeq, Oct 2010]
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Cell cycle