ALPK1 (NM_025144) Human Mass Spec Standard

CAT#: PH309189

ALPK1 MS Standard C13 and N15-labeled recombinant protein (NP_079420)

Product Images

Coomassie blue staining of purified ALPK1 protein (Cat# TP309189). The protein was produced from HEK293T cells transfected with ALPK1 cDNA clone (Cat# RC209189) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC209189
• Predicted MW: 138.9 kDa
• Protein Sequence:
  Sequence Link

  >Peptide sequence encoded by RC209189
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MNNQKVVAVLLQECKQVLDQLLLEAPDVSEEDKSEDQRCRALLPSELRTLIQEAKEMKWPFVPEKWQYK
  QAVGPEDKTNLKDVIGAGLQQLLASLRASILARDCAAAAAIVFLVDRFLYGLDVSGKLLQVAKGLHKLQ
  PATPIAPQVVIRQARISVNSGKLLKAEYILSSLISNNGATGTWLYRNESDKVLVQSVCIQIRGQILQKL
  GMWYEAAELIWASIVGYLALPQPDKKGLSTSLGILADIFVSMSKNDYEKFKNNPQINLSLLKEFDHHLL
  SAAEACKLAAAFSAYTPLFVLTAVNIRGTCLLSYSSSNDCPPELKNLHLCEAKEAFEIGLLTKRDDEPV
  TGKQELHSFVKAAFGLTTVHRRLHGETGTVHAASQLCKEAMGKLYNFSTSSRSQDREALSQEVMSVIAQ
  VKEHLQVQSFSNVDDRSYVPESFECRLDKLILHGQGDFQKILDTYSQHHTSVCEVFESDCGNNKNEQKD
  AKTGVCITALKTEIKNIDTVSTTQEKPHCQRDTGISSSLMGKNVQRELRRGGRRNWTHSDAFRVSLDQD
  VETETEPSDYSNDEGAVFNKSLSGSQTSSAWSNLSGFSSSASWEEVNYHVDDRSARKEPGKEHLVDTQC
  STALSEELENDREGRAMHSLRSQLHDLSLQEPNNDNLEPSQNQPQQQMPLTPFSPHNTPGIFLAPGAGL
  LEGAPEGIQEVRNMGPRNTSAHSRPSYRSASWSSDSGRPKNIGTHPSVQKEEAFEIIVEFPETNCDVKD
  RQGKEQGEEISERGAGPTFKASPSWVDPEGETAESTEDAPLDFHRVLHNSLGNISMLPCSSFTPNWPVQ
  NPDSRKSGGPVAEQGIDPDASTVDEEGQLLDSTDVPCTNGHGSHRLCILRQPPGQRAETPNSSVSGNIL
  FPVLSEDCTTTEEGNQPGNMLNCSQNSSSSSVWWLKSPAFSSGSSEGDSPWSYLNSSGSSWVSLPGKMR
  KEILEARTLQPDDFEKLLAGVRHDWLFQRLENTGVFKPSQLHRAHSALLLKYSKKSELWTAQETIVYLG
  DYLTVKKKGRQRNAFWVHHLHQEEILGRYVGKDYKEQKGLWHHFTDVERQMTAQHYVTEFNKRLYEQNI
  PTQIFYIPSTILLILEDKTIKGCISVEPYILGEFVKLSNNTKVVKTEYKATEYGLAYGHFSYEFSNHRD
  VVVDLQGWVTGNGKGLIYLTDPQIHSVDQKVFTTNFGKRGIFYFFNNQHVECNEICHRLSLTRPSMEKP
  CT
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
  
  Recombinant protein using RC209189 also available, TP309189
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_079420
• RefSeq Size: 4281
• RefSeq ORF: 3732
• Synonyms: 8430410J10Rik; LAK
• Locus ID: 80216
• UniProt ID: Q96QP1
• Cytogenetics: 4q25
• Summary: This gene encodes an alpha kinase. Mice which were homozygous for disrupted copies of this gene exhibited coordination defects (PMID: 21208416). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]
• Protein Families: Druggable Genome, Protein Kinase