ATP5PF (NM_001003696) Human Mass Spec Standard

CAT#: PH309756

ATP5J MS Standard C13 and N15-labeled recombinant protein (NP_001003696)

Product Images

Coomassie blue staining of purified ATP5PF protein (Cat# TP309756). The protein was produced from HEK293T cells transfected with ATP5PF cDNA clone (Cat# RC209756) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC209756
• Predicted MW: 12.6 kDa
• Protein Sequence:
  Sequence Link

  >RC209756 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MILQRLFRFSSVIRSAVSVHLRRNIGVTAVAFNKELDPIQKLFVDKIREYKSKRQTSGGPVDASSEYQQE
  LERELFKLKQMFGNADMNTFHTFKFEDPKFEVIEKPQA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_001003696
• RefSeq Size: 841
• RefSeq ORF: 324
• Synonyms: ATP5; ATP5A; ATP5J; ATPM; CF6; F6
• Locus ID: 522
• UniProt ID: P18859, Q6IB54, Q6NZ59
• Cytogenetics: 21q21.3
• Summary: 'Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo complex has nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the F6 subunit of the Fo complex. The F6 subunit is required for F1 and Fo interactions. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. This gene has 1 or more pseudogenes. [provided by RefSeq, Feb 2016]'
• Protein Pathways: Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease