KLF2 (NM_016270) Human Mass Spec Standard

CAT#: PH310042

KLF2 MS Standard C13 and N15-labeled recombinant protein (NP_057354)

Product Images

Coomassie blue staining of purified KLF2 protein (Cat# TP310042). The protein was produced from HEK293T cells transfected with KLF2 cDNA clone (Cat# RC210042) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC210042
• Predicted MW: 37.4 kDa
• Protein Sequence:
  Sequence Link

  >RC210042 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MALSEPILPSFSTFASPCRERGLQERWPRAEPESGGTDDDLNSVLDFILSMGLDGLGAEAAPEPPPPPPP
  PAFYYPEPGAPPPYSAPAGGLVSELLRPELDAPPGPALHGRFLLAPPGRLVKAEPPEADGGGGYGCAPGL
  TRGPRGLKREGAPGPAASCMRGPGGRPPPPPDTPPLSPDGPARLPAPGPRASFPPPFGGPGFGAPGPGLH
  YAPPAPPAFGLFDDAAAAAAALGLAPPAARGLLTPPASPLELLEAKPKRGRRSWPRKRTATHTCSYAGCG
  KTYTKSSHLKAHLRTHTGEKPYHCNWDGCGWKFARSDELTRHYRKHTGHRPFQCHLCDRAFSRSDHLALH
  MKRHM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_057354
• RefSeq Size: 1655
• RefSeq ORF: 1065
• Synonyms: LKLF
• Locus ID: 10365
• UniProt ID: Q9Y5W3
• Cytogenetics: 19p13.11
• Summary: This gene encodes a protein that belongs to the Kruppel family of transcription factors. The encoded zinc finger protein is expressed early in mammalian development and is found in many different cell types. The protein acts to bind the CACCC box found in the promoter of target genes to activate their transcription. It plays a role in many processes during development and disease including adipogenesis, embryonic erythropoiesis, epithelial integrity, inflammation and t-cell viability. [provided by RefSeq, Mar 2017]
• Protein Families: Transcription Factors