NUDT15 (NM_018283) Human Mass Spec Standard

CAT#: PH310477

NUDT15 MS Standard C13 and N15-labeled recombinant protein (NP_060753)

Product Images

Coomassie blue staining of purified NUDT15 protein (Cat# TP310477). The protein was produced from HEK293T cells transfected with NUDT15 cDNA clone (Cat# RC210477) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC210477
• Predicted MW: 18.4 kDa
• Protein Sequence:
  Sequence Link

  >RC210477 representing NM_018283
  Red=Cloning site Green=Tags(s)
  
  MTASAQPRGRRPGVGVGVVVTSCKHPRCVLLGKRKGSVGAGSFQLPGGHLEFGETWEECAQRETWEEAAL
  HLKNVHFASVVNSFIEKENYHYVTILMKGEVDVTHDSEPKNVEPEKNESWEWVPWEELPPLDQLFWGLRC
  LKEQGYDPFKEDLNHLVGYKGNHL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_060753
• RefSeq Size: 2022
• RefSeq ORF: 492
• Synonyms: MTH2; NUDT15D
• Locus ID: 55270
• UniProt ID: Q9NV35
• Cytogenetics: 13q14.2
• Summary: This gene encodes an enzyme that belongs to the Nudix hydrolase superfamily. Members of this superfamily catalyze the hydrolysis of nucleoside diphosphates, including substrates like 8-oxo-dGTP, which are a result of oxidative damage, and can induce base mispairing during DNA replication, causing transversions. The encoded enzyme is a negative regulator of thiopurine activation and toxicity. Mutations in this gene result in poor metabolism of thiopurines, and are associated with thiopurine-induced early leukopenia. Multiple pseudogenes of this gene have been identified. [provided by RefSeq, Apr 2016]