GSDME (NM_004403) Human Mass Spec Standard

CAT#: PH310515

DFNA5 MS Standard C13 and N15-labeled recombinant protein (NP_004394)

Product Images

Coomassie blue staining of purified GSDME protein (Cat# TP310515). The protein was produced from HEK293T cells transfected with GSDME cDNA clone (Cat# RC210515) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC210515
• Predicted MW: 54.6 kDa
• Protein Sequence:
  Sequence Link

  >RC210515 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MFAKATRNFLREVDADGDLIAVSNLNDSDKLQLLSLVTKKKRFWCWQRPKYQFLSLTLGDVLIEDQFPSP
  VVVESDFVKYEGKFANHVSGTLETALGKVKLNLGGSSRVESQSSFGTLRKQEVDLQQLIRDSAERTINLR
  NPVLQQVLEGRNEVLCVLTQKITTMQKCVISEHMQVEEKCGGIVGIQTKTVQVSATEDGNVTKDSNVVLE
  IPAATTIAYGVIELYVKLDGLFEFCLLRGKQGGFENKKRIDSVYLDHLVFREFAFIDMPDAAHGISSQDG
  PLSVLKQATLLLERNFHPFAELPEPQQTALSDIFQAVVFDDELLMVLEPVCDDLVSGLSPTVAVLGELKP
  RQQQDLVAFLQLVGCSLQGGCPGPEDAGSKQLFMTAYFLVSALAEMPDSAAALLGTCCKLQIIPTLCHLL
  RALSDDGVSDLEDPTLTPLKDTERFGIVQRLFASADISLERLKSSVKAVILKDSKVFPLLLCITLNGLCA
  LGREHS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_004394
• RefSeq Size: 2521
• RefSeq ORF: 1488
• Synonyms: DFNA5; ICERE-1
• Locus ID: 1687
• UniProt ID: O60443, A0A024RA58
• Cytogenetics: 7p15.3
• Summary: 'Hearing impairment is a heterogeneous condition with over 40 loci described. The protein encoded by this gene is expressed in fetal cochlea, however, its function is not known. Nonsyndromic hearing impairment is associated with a mutation in this gene. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'
• Protein Families: Druggable Genome