MAT2B (NM_013283) Human Mass Spec Standard

CAT#: PH310589

MAT2B MS Standard C13 and N15-labeled recombinant protein (NP_037415)

Product Images

Coomassie blue staining of purified MAT2B protein (Cat# TP310589). The protein was produced from HEK293T cells transfected with MAT2B cDNA clone (Cat# RC210589) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC210589
• Predicted MW: 37.5 kDa
• Protein Sequence:
  Sequence Link

  >RC210589 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVGREKELSIHFVPGSCRLVEEEVNIPNRRVLVTGATGLLGRAVHKEFQQNNWHAVGCGFRRARPKFEQV
  NLLDSNAVHHIIHDFQPHVIVHCAAERRPDVVENQPDAASQLNVDASGNLAKEAAAVGAFLIYISSDYVF
  DGTNPPYREGDIPAPLNLYGKTKLDGEKAVLENNLGAAVLRIPILYGEVEKLEESAVTVMFDKVQFSNKS
  ANMDHWQQRFPTHVKDVATVCRQLAEKRMLDPSIKGTFHWSGNEQMTKYEMACAIADAFNLPSSHLRPIT
  DSPVLGAQRPRNAQLDCSKLETLGIGQRTPFRIGIKESLWPFLIDKRWRQTVFH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_037415
• RefSeq Size: 2154
• RefSeq ORF: 1002
• Synonyms: MAT-II; MATIIbeta; Nbla02999; SDR23E1; TGR
• Locus ID: 27430
• UniProt ID: Q9NZL9, A0A140VJP2
• Cytogenetics: 5q34
• Summary: The protein encoded by this gene belongs to the methionine adenosyltransferase (MAT) family. MAT catalyzes the biosynthesis of S-adenosylmethionine from methionine and ATP. This protein is the regulatory beta subunit of MAT. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2012]
• Protein Pathways: Cysteine and methionine metabolism, Metabolic pathways, Selenoamino acid metabolism