CSPS (SULT1A3) (NM_003166) Human Mass Spec Standard

CAT#: PH310772

SULT1A3 MS Standard C13 and N15-labeled recombinant protein (NP_003157)

Product Images

Coomassie blue staining of purified SULT1A3 protein (Cat# TP310772). The protein was produced from HEK293T cells transfected with SULT1A3 cDNA clone (Cat# RC210772) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC210772
• Predicted MW: 34.3 kDa
• Protein Sequence:
  Sequence Link

  >RC210772 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MELIQDTSRPPLEYVKGVPLIKYFAEALGPLQSFQARPDDLLINTYPKSGTTWVSQILDMIYQGGDLEKC
  NRAPIYVRVPFLEVNDPGEPSGLETLKDTPPPRLIKSHLPLALLPQTLLDQKVKVVYVARNPKDVAVSYY
  HFHRMEKAHPEPGTWDSFLEKFMAGEVSYWSWYQHVQEWWELSRTHPVLYLFYEDMKENPKREIQKILEF
  VGRSLPEETMDFMVQHTSFKEMKKNPMTNYTTVPQELMDHSISPFMRKGMAGDWKTTFTVAQNERFDADY
  AEKMAGCSLSFRSEL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_003157
• RefSeq Size: 1604
• RefSeq ORF: 885
• Synonyms: HAST; HAST3; M-PST; MGC117469; ST1A5; STM; SULT1A4; TL-PST
• Locus ID: 6818
• Cytogenetics: 16p11.2
• Summary: 'Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and substrate specificities. The gene structure (number and length of exons) is similar among family members. This gene encodes a phenol sulfotransferase with thermolabile enzyme activity. Four sulfotransferase genes are located on the p arm of chromosome 16; this gene and SULT1A4 arose from a segmental duplication. This gene is the most centromeric of the four sulfotransferase genes. Read-through transcription exists between this gene and the upstream SLX1A (SLX1 structure-specific endonuclease subunit homolog A) gene that encodes a protein containing GIY-YIG domains. [provided by RefSeq, Nov 2010]'
• Protein Pathways: Sulfur metabolism