NEK7 (NM_133494) Human Mass Spec Standard

CAT#: PH311857

NEK7 MS Standard C13 and N15-labeled recombinant protein (NP_598001)

Product Images

Coomassie blue staining of purified NEK7 protein (Cat# TP311857). The protein was produced from HEK293T cells transfected with NEK7 cDNA clone (Cat# RC211857) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC211857
• Predicted MW: 34.4 kDa
• Protein Sequence:
  Sequence Link

  >RC211857 representing NM_133494
  Red=Cloning site Green=Tags(s)
  
  MDEQSQGMQGPPVPQFQPQKALRPDMGYNTLANFRIEKKIGRGQFSEVYRAACLLDGVPVALKKVQIFDL
  MDAKARADCIKEIDLLKQLNHPNVIKYYASFIEDNELNIVLELADAGDLSRMIKHFKKQKRLIPERTVWK
  YFVQLCSALEHMHSRRVMHRDIKPANVFITATGVVKLGDLGLGRFFSSKTTAAHSLVGTPYYMSPERIHE
  NGYNFKSDIWSLGCLLYEMAALQSPFYGDKMNLYSLCKKIEQCDYPPLPSDHYSEELRQLVNMCINPDPE
  KRPDVTYVYDVAKRMHACTASS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_598001
• RefSeq Size: 1072
• RefSeq ORF: 906
• Synonyms: NIMA (never in mitosis gene a)-related kinase 7; NIMA-related kinase 7; OTTHUMP00000033680
• Locus ID: 140609
• UniProt ID: Q8TDX7, B2R8K8
• Cytogenetics: 1q31.3
• Summary: NIMA-related kinases share high amino acid sequence identity with the gene product of the Aspergillus nidulans 'never in mitosis A' gene, which controls initiation of mitosis. [supplied by OMIM, Jul 2002]
• Protein Families: Druggable Genome, Protein Kinase