SHISA3 (NM_001080505) Human Mass Spec Standard

CAT#: PH312334

SHISA3 MS Standard C13 and N15-labeled recombinant protein (NP_001073974)

Product Images

Coomassie blue staining of purified SHISA3 protein (Cat# TP312334). The protein was produced from HEK293T cells transfected with SHISA3 cDNA clone (Cat# RC212334) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC212334
• Predicted MW: 25.7 kDa
• Protein Sequence:
  Sequence Link

  >RC212334 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRALLALCLLLGCLRWGPAGAQQSGEYCHGWVDVQGNYHEGFQCPEDFDTLDATICCGSCALRYCCAAAD
  ARLEQGGCTNDRRELEHPGITAQPVYVPFLIVGSIFIAFIILGSVVAIYCCTCLRPKEPSQQPIRFSLRS
  YQTETLPMILTSTSPRAPSRQSSTATSSSSTGGSIRRFSFARAEPGCLVPSPPPPYTTSHSIHLAQPSGF
  LVSPQYFAYPLQQEPPLPGKSCPDFSSS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_001073974
• RefSeq Size: 1971
• RefSeq ORF: 714
• Synonyms: hShisa3
• Locus ID: 152573
• UniProt ID: A0PJX4
• Cytogenetics: 4p13
• Summary: This gene encodes a single-transmembrane protein which is one of nine members of a family of transmembrane adaptors that modulate both WNT and FGF signaling by blocking the maturation and transport of their receptors to the cell surface. Members of this family contain an N-terminal cysteine-rich domain with a distinct cysteine pattern, a single transmembrane domain, and a C-terminal proline-rich, low complexity region. The encoded protein acts as a tumor suppressor by accelerating beta-catenin degradation. [provided by RefSeq, Jul 2017]
• Protein Families: Transmembrane