AGXT (NM_000030) Human Mass Spec Standard

CAT#: PH312899

AGXT MS Standard C13 and N15-labeled recombinant protein (NP_000021)

Product Images

Coomassie blue staining of purified AGXT protein (Cat# TP312899). The protein was produced from HEK293T cells transfected with AGXT cDNA clone (Cat# RC212899) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC212899
• Predicted MW: 43 kDa
• Protein Sequence:
  Sequence Link

  >RC212899 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MASHKLLVTPPKALLKPLSIPNQLLLGPGPSNLPPRIMAAGGLQMIGSMSKDMYQIMDEIKEGIQYVFQT
  RNPLTLVISGSGHCALEAALVNVLEPGDSFLVGANGIWGQRAVDIGERIGARVHPMTKDPGGHYTLQEVE
  EGLAQHKPVLLFLTHGESSTGVLQPLDGFGELCHRYKCLLLVDSVASLGGTPLYMDRQGIDILYSGSQKA
  LNAPPGTSLISFSDKAKKKMYSRKTKPFSFYLDIKWLANFWGCDDQPRMYHHTIPVISLYSLRESLALIA
  EQGLENSWRQHREAAAYLHGRLQALGLQLFVKDPALRLPTVTTVAVPAGYDWRDIVSYVIDHFDIEIMGG
  LGPSTGKVLRIGLLGCNATRENVDRVTEALRAALQHCPKKKL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_000021
• RefSeq Size: 1611
• RefSeq ORF: 1176
• Synonyms: AGT; AGT1; AGXT1; PH1; SPAT; SPT; TLH6
• Locus ID: 189
• UniProt ID: P21549
• Cytogenetics: 2q37.3
• Summary: This gene is expressed only in the liver and the encoded protein is localized mostly in the peroxisomes, where it is involved in glyoxylate detoxification. Mutations in this gene, some of which alter subcellular targetting, have been associated with type I primary hyperoxaluria. [provided by RefSeq, Jul 2008]
• Protein Families: Druggable Genome
• Protein Pathways: Alanine, aspartate and glutamate metabolism, Glycine, serine and threonine metabolism, Metabolic pathways